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Constructs that reflect differences in variability are of interest to many researchers studying workplace phenomena. The aggregation methods typically used to investigate “variability-based” constructs suffer from several limitations, including the inability to include Level 1 predictors and a failure to account for uncertainty in the variability estimates. We demonstrate how mixed-effects location-scale (MELS) and heterogeneous variance models, which are direct extensions of traditional mixed-effects (or multilevel) models, can be used to test mean (location)- and variability (scale)-related hypotheses simultaneously. The aims of this article are to demonstrate (a) how the MELS and heterogeneous variance models can be estimated with both nested cross-sectional and longitudinal data to answer novel research questions about constructs of interest to organizational researchers, (b) how a Bayesian approach allows for the inclusion of random intercepts and slopes when predicting both variability and mean levels, and finally (c) how researchers can use a multilevel approach to predict between-group heterogeneous variances. In doing so, this article highlights the added value of viewing variability as more than a statistical nuisance in organizational research.